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Journal: Comparative Immunology Reports
Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish
doi: 10.1016/j.cirep.2025.200234
Figure Lengend Snippet: Identification of glycan structures on total hemocytes from Cherax quadricarinatus by immunofluorescence and flow cytometry using lectin staining. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ). Representative scatter plot shows internal complexity (side scatter, SSC) and positive for lectin staining. Immunofluorescences are representative for each lectin staining. Scale bar: 10 µm.
Article Snippet: BA-7801–2, Newark, CA, USA); PNA from
Techniques: Glycoproteomics, Immunofluorescence, Flow Cytometry, Staining
Journal: Comparative Immunology Reports
Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish
doi: 10.1016/j.cirep.2025.200234
Figure Lengend Snippet: Identification of glycan structures by subpopulation of hemocytes from Cherax quadricarinatus by Flow cytometry using lectin staining. Value in each histogram represent mean of percentage of three independent experiments. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA-I (from Sambucus nigra ), MAA-I ( Maackia amurensis ). Red lines represent control assays for specificity recognition using lectins incubated previously with 200 mM specific carbohydrates 30 min before the assays, blue lines are lectins without inhibition.
Article Snippet: BA-7801–2, Newark, CA, USA); PNA from
Techniques: Glycoproteomics, Flow Cytometry, Staining, Control, Incubation, Inhibition
Journal: Comparative Immunology Reports
Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish
doi: 10.1016/j.cirep.2025.200234
Figure Lengend Snippet: Glycan patterns by subpopulation hemocytes after immunostimulation. raw starch microparticles (SMP), Vibrio parahaemolyticus (Vp) and Escherichia coli (Ec). Value in each heatmap represent mean of percentage of three independent experiments. Significant differences are mentioned in the text. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ).
Article Snippet: BA-7801–2, Newark, CA, USA); PNA from
Techniques: Glycoproteomics, Starch